Significant Fraction (significant + fraction)

Distribution by Scientific Domains


Selected Abstracts


Ecological selection maintains cytonuclear incompatibilities in hybridizing sunflowers

ECOLOGY LETTERS, Issue 10 2008
Julianno B. M. Sambatti
Abstract Despite the recent renaissance in studies of ecological speciation, the connection between ecological selection and the evolution of reproductive isolation remains tenuous. We tested whether habitat adaptation of cytoplasmic genomes contributes to the maintenance of reproductive barriers in hybridizing sunflower species, Helianthus annuus and Helianthus petiolaris. We transplanted genotypes of the parental species, reciprocal F1 hybrids and all eight possible backcross combinations of nuclear and cytoplasmic genomes into the contrasting xeric and mesic habitats of the parental species. Analysis of survivorship across two growing seasons revealed that the parental species' cytoplasms were strongly locally adapted and that cytonuclear interactions (CNIs) significantly affected the fitness and architecture of hybrid plants. A significant fraction of the CNIs have transgenerational effects, perhaps due to divergence in imprinting patterns. Our results suggest a common means by which ecological selection may contribute to speciation and have significant implications for the persistence of hybridizing species. [source]


Widespread known and novel phosphonate utilization pathways in marine bacteria revealed by functional screening and metagenomic analyses

ENVIRONMENTAL MICROBIOLOGY, Issue 1 2010
Asuncion Martinez
Summary Phosphonates (Pn), compounds with a direct C,P bond instead of the more common C,O,P ester bond, constitute a significant fraction of marine dissolved organic phosphorus and recent evidence suggests that they may be an alternative source of P for marine microorganisms. To further characterize the microorganisms and pathways involved in Pn utilization, we screened bacterioplankton genomic libraries for their ability to complement an Escherichia coli strain unable to use Pns as a P source. Using this approach we identified a phosphonatase pathway as well as a novel pair of genes that allowed utilization of 2-aminoethylphosphonate (2-AEPn) as the sole P source. These pathways are present in diverse bacteria common in marine plankton including representatives of Proteobacteria, Planctomycetes and Cyanobacteria. Analysis of metagenomic databases for Pn utilization genes revealed that they are widespread and abundant among marine bacteria, suggesting that Pn metabolism is likely to play an important role in P-depleted surface waters, as well as in the more P-rich deep-water column. [source]


Use of stable isotope-labelled cells to identify active grazers of picocyanobacteria in ocean surface waters

ENVIRONMENTAL MICROBIOLOGY, Issue 2 2009
Jorge Frias-Lopez
Summary Prochlorococcus and Synechococcus are the two most abundant marine cyanobacteria. They represent a significant fraction of the total primary production of the world oceans and comprise a major fraction of the prey biomass available to phagotrophic protists. Despite relatively rapid growth rates, picocyanobacterial cell densities in open-ocean surface waters remain fairly constant, implying steady mortality due to viral infection and consumption by predators. There have been several studies on grazing by specific protists on Prochlorococcus and Synechococcus in culture, and of cell loss rates due to overall grazing in the field. However, the specific sources of mortality of these primary producers in the wild remain unknown. Here, we use a modification of the RNA stable isotope probing technique (RNA-SIP), which involves adding labelled cells to natural seawater, to identify active predators that are specifically consuming Prochlorococcus and Synechococcus in the surface waters of the Pacific Ocean. Four major groups were identified as having their 18S rRNA highly labelled: Prymnesiophyceae (Haptophyta), Dictyochophyceae (Stramenopiles), Bolidomonas (Stramenopiles) and Dinoflagellata (Alveolata). For the first three of these, the closest relative of the sequences identified was a photosynthetic organism, indicating the presence of mixotrophs among picocyanobacterial predators. We conclude that the use of RNA-SIP is a useful method to identity specific predators for picocyanobacteria in situ, and that the method could possibly be used to identify other bacterial predators important in the microbial food-web. [source]


Interaction between halogenated aromatic compounds in the Ah receptor signal transduction pathway

ENVIRONMENTAL TOXICOLOGY, Issue 5 2004
Guosheng Chen
Abstract Many toxic and biochemical responses to halogenated aromatic compounds (HACs) such as polychlorinated biphenyls (PCBs) and polychlorinated dibenzo- p -dioxins (PCDDs) are mediated through the aryl hydrocarbon receptor (AhR), which is an intracellular cytosolic target for HACs. Environmental exposure to HACs almost always involves complex mixtures of congeners, some of which can antagonize the action of potent HACs such as 2,3,7,8-tetrachlorodibenzo- p -dioxin (TCDD). In this work we studied TCDD and representative PCB congeners, alone and in mixture, for their effect on CYP1A gene transcription and protein levels in primary rat hepatocytes. Together with our previous work, our results suggest that formation of the Ah receptor-ligand-DRE (dioxin response element) complex is the principal point of divergence in the mechanism between an AhR agonist and an AhR antagonist. The coplanar PCBs 77 and 126 and the mono- ortho PCB 156 were full agonists toward CYP1A1 gene transcription and CYP1A protein levels, showing typical additive behavior with TCDD to the target molecule AhR. In contrast, the nonplanar PCB 153 antagonized the action of TCDD, even at concentrations that occupied a significant fraction of AhR molecules. Competitive inhibition explains the commonly reported decrease of ethoxyresorufin- O -deethylase (EROD) activity when PCBs are present in high concentrations and the antagonism of PCBs to the EROD activity of TCDD. The result is that Western blotting offers a much more reliable measure of CYP1A protein concentration than does the EROD assay, despite the greater convenience of the latter. 2004 Wiley Periodicals, Inc. Environ Toxicol 19: 480,489, 2004. [source]


Why Do Firms Raise Foreign Currency Denominated Debt?

EUROPEAN FINANCIAL MANAGEMENT, Issue 4 2001
Evidence from Finland
This study examines the determinants of the decision to raise currency debt. The results suggest that hedging figures importantly in the currency,of,denomination decision: firms in which exports constitute a significant fraction of net sales are more likely to raise currency debt. However, firms also tend to borrow in periods when the nominal interest rate for the loan currency, relative to other currencies, is lower than usual. This is consistent with the currency debt issue decision being affected by speculative motives. Large firms, with a wider access to the international capital markets, are more likely to borrow in foreign currencies than small firms. [source]


Calcium and Fos Involvement in Brain-Derived Ca2+ -Binding Protein (S100)-Dependent Apoptosis in Rat Phaeochromocytoma Cells

EXPERIMENTAL PHYSIOLOGY, Issue 3 2000
Stefania Fulle
Brain-derived calcium-binding protein S100 induces apoptosis in a significant fraction of rat phaeochromocytoma (PC12) cells. We used single cell techniques (patch clamp, videomicroscopy and immunocytochemistry) to clarify some of the specific aspects of S100-induced apoptosis, the modality(ies) of early intracellular Ca2+ concentration increase and the expression of some classes of genes (c-fos, c-jun, bax, bcl-x, p-15, p-21) known to be implicated in apoptosis of different cells. The results show that S100: (1) causes an increase of [Ca2+]i due to an increased conductance of L-type Ca2+ channels; (2) induces a sustained increase of the Fos levels which is evident since the first time point tested (3 h) and remains elevated until to the last time point (72 h). All these data suggest that S100-derived apoptosis in PC12 cells may be the consequence of a system involving an increase in L-type Ca2+ channel conductance with consequent [Ca2+]i increase which up-regulates, directly or indirectly, the expression of Fos. [source]


Solar Energy Collectors with Tunable Transmission

ADVANCED FUNCTIONAL MATERIALS, Issue 9 2010
Michael G. Debije
Abstract A new type of "smart" window is proposed that makes use of fluorescent dye guests in a liquid-crystal host sandwiched between glass panels. The dye absorbs a variable amount of light depending on its orientation, and re-emits this light, of which a significant fraction is trapped by total internal reflection at the glass,air interface, and becomes concentrated along the edges. Such a device could both generate electricity via an attached photovoltaic as well as allow user control of the amount of transmitted light. By applying a voltage across the cell, absorption could be varied 31%, while the usable light output only varied 11% due to the increased efficiency of light collection at homeotropic dye orientation. [source]


A geomorphic template for the analysis of lake districts applied to the Northern Highland Lake District, Wisconsin, U.S.A.

FRESHWATER BIOLOGY, Issue 3 2000
JoaN. L. Riera
1. We tested the degree to which a lake's landscape position constrains the expression of limnological features and imposes a characteristic spatial pattern in a glacial lake district, the Northern Highland Lake District in north-central Wisconsin. 2. We defined lake order as a metric to analyze the effect of landscape position on limnological features. Lake order, analogous to stream order, is based solely on geographical information and is simple to measure. 3. We examined the strength of the relationship between lake order and a set of 25 variables, which included measures of lake morphometry, water optical properties, major ions, nutrients, biology, and human settlement patterns. 4. Lake order explained a significant fraction of the variance of 21 of the 25 variables tested with ANOVA. The fraction of variance explained varied from 12% (maximum depth) to 56% (calcium concentration). The variables most strongly related to lake order were: measures of lake size and shape, concentrations of major ions (except sulfate) and silica, biological variables (chlorophyll concentration, crayfish abundance, and fish species richness), and human-use variables (density of cottages and resorts). Lake depth, water optical properties, and nutrient concentrations (other than silica) were poorly associated with lake order. 5. Potential explanations for a relationship with lake order differed among variables. In some cases, we could hypothesize a direct link. For example, major ion concentration is a function of groundwater input, which is directly related to lake order. We see these as a direct influence of the geomorphic template left by the retreat of the glacier that led to the formation of this lake district. 6. In other cases, a set of indirect links was hypothesized. For example, the effect of lake order on lake size, water chemistry, and lake connectivity may ultimately explain the relation between lake order and fish species richness. We interpret these relationships as the result of constraints imposed by the geomorphic template on lake development over the last 12 000 years. 7. By identifying relationships between lake characteristics and a measure of landscape position, and by identifying geomorphologic constraints on lake features and lake evolution, our analysis explains an important aspect of the spatial organization of a lake district. [source]


APC/CTNNB1 (,-catenin) pathway alterations in human prostate cancers

GENES, CHROMOSOMES AND CANCER, Issue 1 2002
Amy V. Gerstein
Genetic alterations serve as beacons for the involvement of specific pathways in tumorigenesis. It was previously shown that 5% of prostate tumors harbor CTNNB1 mutations, suggesting that this tumor type may involve a deregulated APC/CTNNB1 pathway. To explore this possibility further, we searched for mutations in genes implicated in this pathway in 22 samples that included cell lines, xenografts, and primary tumors. We identified seven alterations: two in CTNNB1, three in APC, and two in hTRCP1 (also known as BTRC) which controls the degradation of CTNNB1. Alterations in the CTNNB1 regulatory domain, APC, and hTRCP1 were mutually exclusive, consistent with their equivalent effects on CTNNB1 stability. These results suggest that CTNNB1 signaling plays a critical role in the development of a significant fraction of prostate cancers. Moreover, they provide the first evidence that hTRCP1 plays a role in human neoplasia. 2002 Wiley-Liss, Inc. [source]


Soil,atmosphere exchange of CH4, CO, N2O and NOx and the effects of land-use change in the semiarid Mallee system in Southeastern Australia

GLOBAL CHANGE BIOLOGY, Issue 9 2010
IAN GALBALLY
Abstract The semiarid and arid zones cover a quarter of the global land area and support one-fifth of the world's human population. A significant fraction of the global soil,atmosphere exchange for climatically active gases occurs in semiarid and arid zones yet little is known about these exchanges. A study was made of the soil,atmosphere exchange of CH4, CO, N2O and NOx in the semiarid Mallee system, in north-western Victoria, Australia, at two sites: one pristine mallee and the other cleared for approximately 65 years for farming (currently wheat). The mean ( standard error) rates of CH4 exchange were uptakes of ,3.0 0.5 ng(C) m,2 s,1 for the Mallee and ,6.0 0.3 ng(C) m,2 s,1 for the Wheat. Converting mallee forest to wheat crop increases CH4 uptake significantly. CH4 emissions were observed in the Mallee in summer and were hypothesized to arise from termite activity. We find no evidence that in situ growing wheat plants emit CH4, contrary to a recent report. The average CO emissions of 10.1 1.8 ng(C) m,2 s,1 in the Mallee and 12.6 2.0 ng(C) m,2 s,1 in the Wheat. The average N2O emissions were 0.5 0.1 ng(N) m,2 s,1 from the pristine Mallee and 1.4 0.3 ng(N) m,2 s,1 from the Wheat. The experimental results show that the processes controlling these exchanges are different to those in temperate systems and are poorly understood. [source]


Survey augmentation using commercial vessels in the Mid-Atlantic Bight: Sampling density and relative catchability

JOURNAL OF APPLIED ICHTHYOLOGY, Issue 6 2006
E. N. Powell
Summary A series of side-by-side tows was conducted between a survey vessel and a commercial vessel in two seasons, spring and fall (autumn), to examine the use of commercial vessels to increase sampling density in trawl-based stock surveys. Both vessels caught more fish offshore in the spring. The commercial vessel caught more fish than the survey vessel in both seasons. Catches of nearly all species were contagiously distributed in the spring. Most were contagiously distributed in the fall; however, somewhat more species were characterized by random or even distributions. The variance-to-mean ratio was consistently higher for most species for commercial vessel catches, regardless of season. As both vessels sampled in the same region at the same time, the increased predilection for the survey vessel to assess the distribution pattern as less patchy than the commercial vessel must accrue from some difference in sampling dynamics rather than variation in species distribution. A simulated decrease in sampling effort from 59 to 30 or 15 hauls increased the variance-to-mean ratio. Reduced sampling effort increased the tendency for occasional large catches to vary the estimate of domain biomass. The sampling program included an onshore,offshore gradient in station density. Domain biomass was considerably underestimated with reduced station density for six species characterized by large catches offshore in that portion of the survey domain characterized by low station density. In this study, a factor of two variation in domain biomass became more likely in 40% of species when sampling effort was reduced to 15 hauls from 59. A factor of two in biomass may distinguish a sustainable fishery from one in which a species is overfished. As survey sampling effort in this area was 18 hauls, increasing sample number by inclusion of commercial vessel tows would be advantageous. A regression between paired tows failed to adequately predict catches of one vessel from catches of the other. Standardization of vessel catches by the ratio-of-mean catches provided a more realistic comparison because large catches accounted for a significant fraction of domain biomass; however, a single conversion coefficient between boats could not be used for both sampling periods. The underlying impediment in developing a general conversion factor between the two vessels seems to be rooted in the differential in variance-to-mean ratios of the catches; this differential exists despite sampling of the same distribution of fish. [source]


Intracellular redistribution and modification of proteins of the Mre11/Rad50/Nbs1 DNA repair complex following irradiation and heat-shock

JOURNAL OF CELLULAR PHYSIOLOGY, Issue 2 2004
Joshua D. Seno
Mre11, Rad50, and Nbs1form a tight complex which is homogeneously distributed throughout the nuclei of mammalian cells. However, after irradiation, the Mre11/Rad50/Nbs1 (M/R/N) complex rapidly migrates to sites of double strand breaks (DSBs), forming foci which remain until DSB repair is complete. Mre11 and Rad50 play direct roles in DSB repair, while Nbs1 appears to be involved in damage signaling. Hyperthermia sensitizes mammalian cells to ionizing radiation. Radiosensitization by heat shock is believed to be mediated by an inhibition of DSB repair. While the mechanism of inhibition of repair by heat shock remains to be elucidated, recent reports suggest that the M/R/N complex may be a target for inhibition of DSB repair and radiosensitization by heat. We now demonstrate that when human U-1 melanoma cells are heated at 42.5 or 45.5C, Mre11, Rad50, and Nbs1 are rapidly translocated from the nucleus to the cytoplasm. Interestingly, when cells were exposed to ionizing radiation (12 Gy of X-rays) prior to heat treatment, the extent and kinetics of translocation were increased when nuclear and cytoplasmic fractions of protein were analyzed immediately after treatment. The kinetics of the translocation and subsequent relocalization back into the nucleus when cells were incubated at 37C from 30 min to 7 h following treatment were different for each protein, which suggests that the proteins redistribute independently. However, a significant fraction of the translocated proteins exist as a triple complex in the cytoplasm. Treatment with leptomycin B (LMB) inhibits the translocation of Mre11, Rad50, and Nbs1 to the cytoplasm, leading us to speculate that the relocalization of the proteins to the cytoplasm occurs via CRM1-mediated nuclear export. In addition, while Nbs1 is rapidly phosphorylated in the nuclei of irradiated cells and is critical for a normal DNA damage response, we have found that Nbs1 is rapidly phosphorylated in the cytoplasm, but not in the nucleus, of heated irradiated cells. The phosphorylation of cytoplasmic Nbs1, which cannot be inhibited by wortmannin, appears to be a unique post-translational modification in heated, irradiated cells, and coupled with our novel observations that Mre11, Rad50, and Nbs1 translocate to the cytoplasm, lend further support for a role of the M/R/N complex in thermal radiosensitization and inhibition of DSB repair. J. Cell. Physiol. 199: 157,170, 2004 2004 Wiley-Liss, Inc. [source]


Optimized virus disruption improves detection of HIV-1 p24 in particles and uncovers a p24 reactivity in patients with undetectable HIV-1 RNA under long-term HAART

JOURNAL OF MEDICAL VIROLOGY, Issue 8 2006
Jrg Schpbach
Abstract HIV-1 p24 antigen (p24) measurement by signal amplification-boosted ELISA of heat-denatured plasma is being evaluated as an alternative to HIV-1 RNA quantitation in resource-poor settings. Some observations suggested that virion-associated p24 is suboptimally detected using Triton X-100-based virus dissociation buffer (kit buffer). A new reagent (SNCR buffer) containing both denaturing and non-denaturing detergents was therefore developed and evaluated. The SNCR buffer increased the measured p24 concentration about 1.5- to 3-fold in HIV-negative plasma reconstituted with purified HIV-1 particles, while not increasing the background. Among 127 samples of HIV-1-positive patients with moderate to high concentrations of HIV-1 RNA the increase was about threefold across the entire concentration range (P,<,0.0001). Specificity before neutralization among prospectively tested clinical samples ruled HIV-negative was 828 of 845 (98.0%) for the SNCR buffer and 464 of 479 (96.9%) for kit buffer. Specificity after confirmatory neutralization of reactive samples or a follow-up test was 100% with either buffer. Surprisingly, the SNCR buffer revealed a p24 reactivity in 115 of 187 samples (61.5%) from adult patients exhibiting undetectable HIV-1 RNA below 5 copies/ml for a duration of 6,30 months under HAART (3.7% with kit buffer). The rate of p24 reactivity in these patients did not decrease with duration of HAART. In conclusion, the SNCR buffer improves the detection of particle-associated HIV-1 p24, thereby increasing the measured p24 concentration in samples with medium to high HIV-1 RNA. It also uncovers the presence of a p24 reactivity, whose identity remains to be determined, in a significant fraction of samples with undetectable HIV-1 RNA under long-term HAART. J. Med. Virol. 78:1003,1010, 2006. 2006 Wiley-Liss, Inc. [source]


Schlieren formation in diatexite migmatite: examples from the St Malo migmatite terrane, France

JOURNAL OF METAMORPHIC GEOLOGY, Issue 4 2003
I. Milord
Abstract Schlieren are trains of platy or blocky minerals, typically the ferromagnesian minerals and accessory phases, that occur in granites and melt-rich migmatites, such as diatexites. They have been considered as: (1) unmelted residue from xenoliths or the source region; (2) mineral accumulations formed during magma flow; (3) compositional layering; and (4) sites of melt loss. In order to help identify schlieren-forming processes in the diatexites at St Malo, differences in the size, shape, orientation, distribution and composition of the biotite from schlieren and from their hosts have been investigated. Small biotite grains are much less abundant in the schlieren than in their hosts. Schlieren biotite grains are generally larger, have greater aspect ratios and have, except in hosts with low (< 10%) biotite contents, a much stronger shape preferred orientation than host biotite. The compositional ranges of host and schlieren biotite are similar, but schlieren biotite defines tighter, sharper peaks on composition-frequency plots. Hosts show magmatic textures such as imbricated (tiled), unstrained plagioclase. Some schlieren show only magmatic textures (tiled biotite, no crystal-plastic strain features), but many have textures indicating submagmatic and subsolidus deformation (e.g. kinked grains) and these schlieren show the most extensive evidence for recrystallization. Magmas at St Malo initially contained a significant fraction of residual biotite and plagioclase crystals; smaller biotite grains were separated from the larger plagioclase crystals during magma flow. Since plagioclase was also the major, early crystallizing phase, the plagioclase-rich domains developed rapidly and reached the rigid percolation threshold first, forcing further magma flow to be concentrated into narrowing melt-rich zones where the biotite had accumulated, hence increasing shear strain and the degree of shape preferred orientation in these domains. Schlieren formed in these domains as a result of grain contacts and tiling in the grain inertia-regime. Final amalgamation of the biotite aggregates into schlieren involved volume loss as melt trapped between grains was expelled after the rigid percolation threshold was reached in the biotite-rich layers. [source]


Skin permeability enhancement by low frequency sonophoresis: Lipid extraction and transport pathways

JOURNAL OF PHARMACEUTICAL SCIENCES, Issue 6 2003
R. Alvarez-Romn
Abstract The objective of this study was to shed light on the mechanism(s) by which low-frequency ultrasound (20 KHz) enhances the permeability of the skin. The physical effects on the barrier and the transport pathway, in particular, were examined. The amount of lipid removed from the intercellular domains of the stratum corneum following sonophoresis was determined by infrared spectroscopy. Transport of the fluorescent probes nile red and calcein, under the influence of ultrasound, was evaluated by laser-scanning confocal microscopy. The results were compared with the appropriate passive control data and with data obtained from experiments in which the skin was exposed simply to the thermal effects induced by ultrasound treatment. A significant fraction (,30%) of the intercellular lipids of the stratum corneum, which are principally responsible for skin barrier function, were removed during the application of low-frequency sonophoresis. Although the confocal images from the nile red experiments were not particularly informative, ultrasound clearly and significantly (again, relative to the corresponding controls) facilitated transport of the hydrophilic calcein via discrete permeabilized regions, whereas other areas of the barrier were apparently unaffected. Lipid removal from the stratum corneum is implicated as a factor contributing the observed permeation enhancement effects of low-frequency ultrasound. However, microscopic observations imply that sonophoresis induces localized (aqueous?) permeation pathways at discrete sites. 2003 Wiley-Liss, Inc. and the American Pharmaceutical Association J Pharm Sci 92:1138,1146, 2003 [source]


HYDROLOGIC MODELING OF A BIOINFILTRATION BEST MANAGEMENT PRACTICE,

JOURNAL OF THE AMERICAN WATER RESOURCES ASSOCIATION, Issue 5 2006
William Heasom
ABSTRACT: The goal of this research was to develop a methodology for modeling a bioinfiltration best management practice (BMP) built in a dormitory area on the campus of Villanova University in Pennsylvania. The objectives were to quantify the behavior of the BMP through the different seasons and rainfall events; better understand the physical processes governing the system's behavior; and develop design criteria. The BMP was constructed in 2001 by excavating within an existing traffic island, backfilling with a sand/soil mixture, and planting with salt tolerant grasses and shrubs native to the Atlantic shore. It receives runoff from the asphalt (0.26 hectare) and turf (0.27 hectare) surfaces of the watershed. Monitoring supported by the hydrologic model shows that the facility infiltrates a significant fraction of the annual precipitation, substantially reducing the delivery of nonpoint source pollution and erosive surges downstream. A hydrologic model was developed using HEC-HMS to represent the site and the BMP using Green-Ampt and kinematic wave methods. Instruments allow comparison of the modeled and measured water budget parameters. The model, incorporating seasonally variable parameters, predicts the volumes infiltrated and bypassed by the BMP, confirming the applicability of the selected methods for the analysis of bioinfiltration BMPs. [source]


Conformational anti-cytochrome P4502E1 (CYP2E1) auto-antibodies contribute to necro-inflammatory injury in chronic hepatitis C

JOURNAL OF VIRAL HEPATITIS, Issue 10 2010
S. Sutti
Summary., Circulating auto-antibodies against cytochrome P4502E1 (CYP2E1) have been observed in a significant fraction of patients with chronic hepatitis C (CHC). This study investigated the clinical significance of these auto-antibodies in relation to their antigen specificity. The presence of anti-CYP2E1 IgG was investigated in 137 consecutive patients with biopsy-proven CHC. Anti-CYP2E1 IgG above control threshold levels was detected in 52 (38%) subjects. By combined immunoprecipitation and western blotting, we observed that among anti-CYP2E1 IgG-positive sera, 23 (44%) were unreactive towards denaturated CYP2E1, indicating a prevalent recognition of conformational CYP2E1 antigens. Conformational anti-CYP2E1 auto-antibodies were unrelated to circulating gamma-globulins, alcohol intake or infection by specific HCV genotypes. The presence of anti-CYP2E1 auto-antibodies was associated with an 11-fold (OR 10.9 95%CI 1.4,86.6 P = 0.008) increased prevalence of necro-inflammatory grading ,4 (Ishack's criteria) and 4-fold (OR 4.0; 95%CI 1.3-11-7: P = 0.014) increased prevalence of fibrosis staging ,2, respectively. Multivariate analysis confirmed conformational anti-CYP2E1 IgG (P = 0.005) and age (P = 0.033) as independent predictors of necro-inflammatory grading ,4. The development of anti-CYP2E1 auto-antibodies targeting conformational CYP2E1 epitopes is associated with more severe liver damage in CHC. [source]


Conserving the evolutionary potential of California valley oak (Quercus lobata Ne): a multivariate genetic approach to conservation planning

MOLECULAR ECOLOGY, Issue 1 2008
DELPHINE GRIVET
Abstract California valley oak (Quercus lobata Ne) is a seriously threatened endemic oak species in California and a keystone species for foothill oak ecosystems. Urban and agricultural development affects a significant fraction of the species' range and predicted climate change is likely to dislocate many current populations. Here, we explore spatial patterns of multivariate genotypes and genetic diversity throughout the range of valley oak to determine whether ongoing and future patterns of habitat loss could threaten the evolutionary potential of the species by eradicating populations of distinctive genetic composition. This manuscript will address three specific questions: (i) What is the spatial genetic structure of the chloroplast and nuclear genetic markers? (ii) What are the geographical trends in the distribution of chloroplast and nuclear genotypes? (iii) Is there any part of the species' range where allelic diversity in either the chloroplast or nuclear genomes is particularly high? We analysed six chloroplast and seven nuclear microsatellite genetic markers of individuals widespread across the valley oak range. We then used a multivariate approach correlating genetic markers and geographical variables through a canonical trend surface analysis, followed by GIS mapping of the significant axes. We visualized population allelic richness spatially with GIS tools to identify regions of high diversity. Our findings, based on the distribution of multivariate genotypes and allelic richness, identify areas with distinctive histories and genetic composition that should be given priority in reserve network design, especially because these areas also overlap with landscape change and little degree of protection. Thus, without a careful preservation plan, valuable evolutionary information will be lost for valley oak. [source]


A microsatellite-based estimation of clonal diversity and population subdivision in Zostera marina, a marine flowering plant

MOLECULAR ECOLOGY, Issue 2 2000
T. B. H. Reusch
Abstract We examined the genetic population structure in eelgrass (Zostera marina L.), the dominant seagrass species of the northern hemisphere, over spatial scales from 12 km to 10 000 km using the polymorphism of DNA microsatellites. Twelve populations were genotyped for six loci representing a total of 67 alleles. Populations sampled included the North Sea (four), the Baltic Sea (three), the western Atlantic (two), the eastern Atlantic (one), the Mediterranean Sea (one) and the eastern Pacific (one). Microsatellites revealed substantial genetic variation in a plant group with low allozyme diversity. Average expected heterozygosities per population (monoclonal populations excluded) ranged from 0.32 to 0.61 (mean = 0.48) and allele numbers varied between 3.3 and 6.7 (mean = 4.7). Using the expected frequency of multilocus genotypes within populations, we distinguished ramets from genetic individuals (i.e. equivalent to clones). Differences in clonal diversity among populations varied widely and ranged from maximal diversity (i.e. all ramets with different genotype) to near or total monoclonality (two populations). All multiple sampled ramets were excluded from further analysis of genetic differentiation within and between populations. All but one population were in Hardy,Weinberg equilibrium, indicating that Zostera marina is predominantly outcrossing. From a regression of the pairwise population differentiation with distance, we obtained an effective population size Ne of 2440,5000. The overall genetic differentiation among eelgrass populations, assessed as , (a standardized estimate of Slatkin's RST) was 0.384 (95% CI 0.34,0.44, P < 0.001). Genetic differentiation was weak among three North Sea populations situated 12,42 km distant from one another, suggesting that tidal currents result in an efficient exchange of propagules. In the Baltic and in Nova Scotia, a small but statistically significant fraction of the genetic variance was distributed between populations (, = 0.029,0.053) at scales of 15,35 km. Pairwise genetic differentiation between European populations were correlated with distance between populations up to a distance of 4500 km (linear differentiation-by-distance model, R2 = 0.67). In contrast, both Nova Scotian populations were genetically much closer to North Sea and Baltic populations than expected from their geographical distance (pairwise , = 0.03,0.08, P < 0.01). A biogeographical cluster of Canadian with Baltic/North Sea populations was also supported using a neighbour-joining tree based on Cavalli,Sforza's chord distance. Relatedness between populations may be very different from predictions based on geographical vicinity. [source]


Modelling CO formation in the turbulent interstellar medium

MONTHLY NOTICES OF THE ROYAL ASTRONOMICAL SOCIETY, Issue 1 2010
S. C. O. Glover
ABSTRACT We present results from high-resolution three-dimensional simulations of turbulent interstellar gas that self-consistently follow its coupled thermal, chemical and dynamical evolution, with a particular focus on the formation and destruction of H2 and CO. We quantify the formation time-scales for H2 and CO in physical conditions corresponding to those found in nearby giant molecular clouds, and show that both species form rapidly, with chemical time-scales that are comparable to the dynamical time-scale of the gas. We also investigate the spatial distributions of H2 and CO, and how they relate to the underlying gas distribution. We show that H2 is a good tracer of the gas distribution, but that the relationship between CO abundance and gas density is more complex. The CO abundance is not well-correlated with either the gas number density n or the visual extinction AV: both have a large influence on the CO abundance, but the inhomogeneous nature of the density field produced by the turbulence means that n and AV are only poorly correlated. There is a large scatter in AV, and hence CO abundance, for gas with any particular density, and similarly a large scatter in density and CO abundance for gas with any particular visual extinction. This will have important consequences for the interpretation of the CO emission observed from real molecular clouds. Finally, we also examine the temperature structure of the simulated gas. We show that the molecular gas is not isothermal. Most of it has a temperature in the range of 10,20 K, but there is also a significant fraction of warmer gas, located in low-extinction regions where photoelectric heating remains effective. [source]


X-ray groups and clusters of galaxies in the Subaru,XMM Deep Field

MONTHLY NOTICES OF THE ROYAL ASTRONOMICAL SOCIETY, Issue 4 2010
A. Finoguenov
Abstract We present the results of a search for galaxy clusters in the Subaru,XMM Deep Field (SXDF). We reach a depth for a total cluster flux in the 0.5,2 keV band of 2 10,15 erg cm,2 s,1 over one of the widest XMM,Newton contiguous raster surveys, covering an area of 1.3 deg2. Cluster candidates are identified through a wavelet detection of extended X-ray emission. The red-sequence technique allows us to identify 57 cluster candidates. We report on the progress with the cluster spectroscopic follow-up and derive their properties based on the X-ray luminosity and cluster scaling relations. In addition, three sources are identified as X-ray counterparts of radio lobes, and in three further sources, an X-ray counterpart of the radio lobes provides a significant fraction of the total flux of the source. In the area covered by near-infrared data, our identification success rate achieves 86 per cent. We detect a number of radio galaxies within our groups, and for a luminosity-limited sample of radio galaxies we compute halo occupation statistics using a marked cluster mass function. We compare the cluster detection statistics in the SXDF with that in the literature and provide the modelling using the concordance cosmology combined with current knowledge of the X-ray cluster properties. The joint cluster log(N) , log(S) is overpredicted by the model, and an agreement can be achieved through a reduction of the concordance ,8 value by 5 per cent. Having considered the dn/dz and the X-ray luminosity function of clusters, we conclude that to pin down the origin of disagreement a much wider (50 deg2) survey is needed. [source]


Observation and modelling of main-sequence star chromospheres , X. Radiative budgets on Gl 867A and AU Mic (dM1e), and a two-component model chromosphere for Gl 205 (dM1),

MONTHLY NOTICES OF THE ROYAL ASTRONOMICAL SOCIETY, Issue 4 2010
E. R. Houdebine
ABSTRACT We report on high-resolution observations of two dM1 stars: Gl 867A, an active dM1e star, and Gl 205, a less active dM1 star. The wavelength coverage is from 3890 to 6820 with a resolving power of about 45 000. The difference spectrum of these two stars allows us to make a survey of spectral lines sensitive to magnetic activity. We chose these two stars because, to within measurement errors, they have very close properties: Gl 867A has R= 0.726 R,, [M/H]= 0.080 dex and Teff= 3416 K, and Gl 205 has R= 0.758 R,, [M/H]= 0.101 dex and Teff= 3493 K. We find that besides traditional chromospheric lines, many photospheric lines are ,filled-in' in the active star spectrum. These differences are, most of the time, weak in absolute fluxes but can be large in terms of differences in the spectral-line equivalent widths. We calculate the differences in surface fluxes between these two stars for many spectral lines. We derive the radiative budgets for two dM1e stars: Gl 867A and AU Mic. We show that the sum of the numerous spectral lines represents a significant fraction of the radiative cooling of the outer atmosphere. We also re-investigate the cooling from the continuum from the visible to the extreme ultraviolet; we find that earlier predictions of the calculations of Houdebine et al. (Paper V) are in good agreement with observations. We emphasize that if this radiative cooling is chromospheric in character, then in chromospheric model calculations, we should include the radiative losses in Ca i, Cr i, V i, Ti i and Fe i. From simple constraints, we derive model chromospheres for quiescent and active regions on Gl 205. We show that the quiescent regions have a strong absorption H, profile. The plage regions show a filled-in intermediate activity H, profile. We also present possible spectral line profiles of quiescent and active regions on Gl 867A. [source]


Why are AGN found in high-mass galaxies?

MONTHLY NOTICES OF THE ROYAL ASTRONOMICAL SOCIETY, Issue 2 2008
Lan Wang
ABSTRACT There is a strong observed mass dependence of the fraction of nearby galaxies that contain either low-luminosity [low-ionization nuclear emission-line region (LINER) type] or higher luminosity (Seyfert or composite type) active galactic nuclei (AGN). This implies that either only a small fraction of low-mass galaxies contain black holes, or that the black holes in these systems only accrete rarely or at very low rates, and hence are generally not detectable as AGN. In this paper, we use semi-analytic models implemented in the Millennium Simulation to analyse the mass dependence of the merging histories of dark matter haloes and of the galaxies that reside in them. Only a few per cent of galaxies with stellar masses less than M* < 1010 M, are predicted to have experienced a major merger. The fraction of galaxies that have experienced major mergers increases steeply at larger stellar masses. We argue that if a major merger is required to form the initial seed black hole, the mass dependence of AGN activity in local galaxies can be understood quite naturally. We then investigate when the major mergers that first create these black holes are predicted to occur. High-mass galaxies are predicted to have formed their first black holes at very early epochs. The majority of low-mass galaxies never experience a major merger and hence may not contain a black hole, but a significant fraction of the supermassive black holes that do exist in low-mass galaxies are predicted to have formed recently. [source]


Where are the cosmic metals at z, 3?

MONTHLY NOTICES OF THE ROYAL ASTRONOMICAL SOCIETY, Issue 1 2008
Jesper Sommer-Larsen
ABSTRACT The global temperature distribution of the cosmic gas-phase oxygen at z, 3 is determined by combining high-resolution cosmological simulations of individual protogalactic as well as larger regions with the observed, extinction-corrected, rest-frame V -band galaxy luminosity function. The simulations have been performed with three different stellar initial mass functions (IMFs), a Kroupa (K98), a Salpeter (S) and an Arimoto,Yoshii (AY), spanning a range of a factor of 5 in chemical yield and specific supernova type II energy feedback. Gas-phase oxygen is binned according to T as log(T) , 4.0 (,cold'), log(T) , 4.5 (,warm') and log(T) , 5.0, 5.5, 6.0, 6.5, 7.0 (,hot' phases). Oxygen is found to be distributed over all T phases, in particular for the top-heavy AY IMF. But, at variance with previous works, it is found that for the K98 and S IMFs the cold phase is the most important. For these IMFs it contains 47 and 37 per cent, respectively, of all gas-phase oxygen, mainly at fairly high density, nH, 0.1 cm,3. The implications of this in relation to observational damped Ly, absorber studies are discussed. In relation to ,missing metals' it is found that a significant fraction of the oxygen is located in a warm/hot phase that may be very difficult to detect. Moreover, it is found that less than about 20,25 per cent of the cosmic oxygen is associated with galaxies brighter than MV,,22, i.e. the faintest galaxy luminosities probed by current metallicity determinations for Lyman-break galaxies (LBGs). Hence, 75,80 per cent of the oxygen is also in this sense ,missing'. From the LBG-based, ,, 1500 ultraviolet luminosity density history at z, 3, we obtain an essentially IMF-independent constraint on the mean oxygen density at z= 3. We compare this to what is obtained from our models, for the three different IMFs. We find that the K98 IMF is strongly excluded, as the chemical yield is simply too small, the Salpeter is marginally excluded, and the AY matches the constraint well. The K98 IMF can only match the data if the ,, 1500 extinction corrections have been overestimated by factor of ,4, which seems highly unlikely. The yields for K98 are also far too small to match the observational data for C iv. The optimal IMF should have a yield intermediate between the S and AY. [source]


Post-common-envelope binaries from SDSS , I. 101 white dwarf main-sequence binaries with multiple Sloan Digital Sky Survey spectroscopy

MONTHLY NOTICES OF THE ROYAL ASTRONOMICAL SOCIETY, Issue 4 2007
A. Rebassa-Mansergas
ABSTRACT We present a detailed analysis of 101 white dwarf main-sequence binaries (WDMS) from the Sloan Digital Sky Survey (SDSS) for which multiple SDSS spectra are available. We detect significant radial velocity variations in 18 WDMS, identifying them as post-common-envelope binaries (PCEBs) or strong PCEB candidates. Strict upper limits to the orbital periods are calculated, ranging from 0.43 to 7880 d. Given the sparse temporal sampling and relatively low spectral resolution of the SDSS spectra, our results imply a PCEB fraction of ,15 per cent among the WDMS in the SDSS data base. Using a spectral decomposition/fitting technique we determined the white dwarf effective temperatures and surface gravities, masses and secondary star spectral types for all WDMS in our sample. Two independent distance estimates are obtained from the flux-scaling factors between the WDMS spectra, and the white dwarf models and main-sequence star templates, respectively. Approximately one-third of the systems in our sample show a significant discrepancy between the two distance estimates. In the majority of discrepant cases, the distance estimate based on the secondary star is too large. A possible explanation for this behaviour is that the secondary star spectral types that we determined from the SDSS spectra are systematically too early by one to two spectral classes. This behaviour could be explained by stellar activity, if covering a significant fraction of the star by cool dark spots will raise the temperature of the interspot regions. Finally, we discuss the selection effects of the WDMS sample provided by the SDSS project. [source]


The SAURON project , VII.

MONTHLY NOTICES OF THE ROYAL ASTRONOMICAL SOCIETY, Issue 2 2006
Integral-field absorption, emission-line kinematics of 24 spiral galaxy bulges
ABSTRACT We present observations of the stellar and gas kinematics for a representative sample of 24 Sa galaxies obtained with our custom-built integral-field spectrograph SAURON operating on the William Herschel Telescope. The data have been homogeneously reduced and analysed by means of a dedicated pipeline. All resulting data cubes were spatially binned to a minimum mean signal-to-noise ratio of 60 per spatial and spectral resolution element. Our maps typically cover the bulge-dominated region. We find a significant fraction of kinematically decoupled components (12/24), many of them displaying central velocity dispersion minima. They are mostly aligned and co-rotating with the main body of the galaxies, and are usually associated with dust discs and rings detected in unsharp-masked images. Almost all the galaxies in the sample (22/24) contain significant amounts of ionized gas which, in general, is accompanied by the presence of dust. The kinematics of the ionized gas are consistent with circular rotation in a disc co-rotating with respect to the stars. The distribution of mean misalignments between the stellar and gaseous angular momenta in the sample suggests that the gas has an internal origin. The [O iii]/H, ratio is usually very low, indicative of current star formation, and shows various morphologies (ring-like structures, alignments with dust lanes or amorphous shapes). The star formation rates (SFRs) in the sample are comparable with that of normal disc galaxies. Low gas velocity dispersion values appear to be linked to regions of intense star formation activity. We interpret this result as stars being formed from dynamically cold gas in those regions. In the case of NGC 5953, the data suggest that we are witnessing the formation of a kinematically decoupled component from cold gas being acquired during the ongoing interaction with NGC 5954. [source]


The nature of high-redshift galaxies

MONTHLY NOTICES OF THE ROYAL ASTRONOMICAL SOCIETY, Issue 4 2001
Rachel S. Somerville
Using semi-analytic models of galaxy formation set within the cold dark matter (CDM) merging hierarchy, we investigate several scenarios for the nature of the high-redshift ) Lyman-break galaxies (LBGs). We consider a ,collisional starburst' model in which bursts of star formation are triggered by galaxy,galaxy mergers, and find that a significant fraction of LBGs are predicted to be starbursts. This model reproduces the observed comoving number density of bright LBGs as a function of redshift and the observed luminosity function at and with a reasonable amount of dust extinction. Model galaxies at have star formation rates, half-light radii, colours and internal velocity dispersions that are in good agreement with the data. Global quantities such as the star formation rate density and cold gas and metal content of the Universe as a function of redshift also agree well. Two ,quiescent' models without starbursts are also investigated. In one, the star formation efficiency in galaxies remains constant with redshift, while in the other, it scales inversely with disc dynamical time, and thus increases rapidly with redshift. The first quiescent model is strongly ruled out, as it does not produce enough high-redshift galaxies once realistic dust extinction is accounted for. The second quiescent model fits marginally, but underproduces cold gas and very bright galaxies at high redshift. A general conclusion is that star formation at high redshift must be more efficient than locally. The collisional starburst model appears to accomplish this naturally without violating other observational constraints. [source]


Stellar contributors to the hard X-ray background?

MONTHLY NOTICES OF THE ROYAL ASTRONOMICAL SOCIETY, Issue 3 2000
Priyamvada Natarajan
We use simple energetic arguments to estimate the contribution of massive X-ray binaries and supernova remnants to the cosmic X-ray background (XRB) at energies in excess of 2 keV. Recent surveys have shown that active galactic nuclei (AGN) probably account for most of the hard XRB (E>2 keV), but there have been many suggestions that star-forming galaxies could emerge at fainter fluxes and perhaps account for a significant fraction of the soft and hard X-ray energy density. Assuming that the formation rate of massive X-ray binaries (MXRBs) traces the global star-formation rate, we find that their integrated contribution to the hard XRB can be estimated and is shown to be small (at less than the 1 per cent level). Similarly, the integrated flux of supernovae (SN) is also shown to be insignificant, or at most comparable to MXRBs. AGN therefore remain the most viable candidates for producing the hard XRB, unless additional processes can be shown to dominate the global hard X-ray emission in distant starburst galaxies. [source]


Stellar dynamical evidence against a cold disc origin for stars in the Galactic Centre

MONTHLY NOTICES OF THE ROYAL ASTRONOMICAL SOCIETY: LETTERS (ELECTRONIC), Issue 1 2008
Jorge Cuadra
ABSTRACT Observations of massive stars within the central parsec of the Galaxy show that, while most stars orbit within a well-defined disc, a significant fraction have large eccentricities and/or inclinations with respect to the disc plane. Here, we investigate whether this dynamically hot component could have arisen via scattering from an initially cold disc , the expected initial condition if the stars formed from the fragmentation of an accretion disc. Using N -body methods, we evolve a variety of flat, cold, stellar systems, and study the effects of initial disc eccentricity, primordial binaries, very massive stars and intermediate mass black holes. We find, consistent with previous results, that a circular disc does not become eccentric enough unless there is a significant population of undetected 100,1000 M, objects. However, since fragmentation of an eccentric disc can readily yield eccentric stellar orbits, the strongest constraints come from inclinations. We show that none of our initial conditions yields the observed large inclinations, regardless of the initial disc eccentricity or the presence of massive objects. These results imply that the orbits of the young massive stars in the Galactic Centre are largely primordial, and that the stars are unlikely to have formed as a dynamically cold disc. [source]


Correlated evolution of fruit and leaf size in bird-dispersed plants: species-level variance in fruit traits explained a bit further?

OIKOS, Issue 3 2002
Carlos M. Herrera
The astounding morphological diversity exhibited by the fruits of vertebrate-dispersed plants has been traditionally interpreted as the adaptive outcome of divergent selective pressures exerted on plants by the broad array of frugivorous animals involved in seed dispersal. Although the selective capacity of frugivores provides support to this interpretation, recent studies have challenged it by documenting a strong phylogenetic component associated to interspecific variation in most fruit characteristics. Size-related fruit traits provide a conspicuous exception to this pattern, because they exhibit considerable variation at the between-species level which is largely independent of phylogeny and is correlated with consumption by differently-sized dispersal agents. Substantial species-level variance in size-related traits may reflect genuine disperser-driven diversification, but may also be partly influenced by correlated evolution of fruit size with the size of other plant structures. This latter possibility is tested here for bird-dispersed plants of the Iberian Peninsula using phylogenetically independent contrasts. Results demonstrate the existence of correlated evolution of fruit and leaf size at the species level. As all the plant taxa considered have their fruits eaten, and seeds dispersed, by the same relatively reduced set of frugivorous bird species, results suggest that a significant fraction of the variation in fruit size represented in the species sample may be explained as an indirect consequence of variation in leaf size, rather than being associated with adaptive divergence related to seed dispersal agents. [source]