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Only One Gene (only + one_gene)
Selected AbstractsBlue light inhibits stomatal development in soybean isolines containing kaempferol-3- O -2G -glycosyl-gentiobioside (K9), a unique flavonoid glycosidePLANT CELL & ENVIRONMENT, Issue 8 2000L. Liu-Gitz ABSTRACT Stomata have a fundamental role in controlling plant photosynthesis and transpiration, but very little is known about factors controlling stomatal differentiation and development. Lines of soybean that contain a specific flavonol glycoside, kaempferol-3- O -2-glycosyl-gentiobioside (K9), as well as greatly reduced stomatal density, especially on the adaxial epidermis, have been identified. The specific effects of blue light photoreceptors on stomatal development in K9 lines and their isoline pairs containing no K9 were studied. Low irradiances of blue light (7% of total photosynthetically active radiation) added to high irradiances from low-pressure sodium lamps strongly inhibited stomatal development on the adaxial epidermis of K9 lines, but not in isoline pairs differing putatively in only one gene and lacking K9. Overall, blue light slightly increased stomatal density on the abaxial epidermis in all isolines, demonstrating differential regulation of stomatal development in the upper and lower epidermis. Blue light also caused an increase in leaf area in all isolines, indicating that changes in stomatal density were not the non-specific result of alterations in leaf area. Morphological studies revealed that the blue light-induced reduction in stomatal density in K9 lines was due to reduced stomatal initiation as well as aborted or abnormal stomatal development. As the phytochrome photostationary state was kept constant, the results indicate that one or more blue light receptors are involved in the control of stomatal development. This system should be useful for the study of mechanisms controlling stomatal development, even if the photo-inhibitory response is unique to K9 lines. [source] A functional genomics approach to evaluate candidate genes located in a QTL interval for milk production traits on BTA6ANIMAL GENETICS, Issue 4 2009P. A. Sheehy Summary The potential genetic and economic advantage of marker-assisted selection for enhanced production in dairy cattle has provided an impetus to conduct numerous genome scans in order to identify associations between DNA markers and future productive potential. One area of focus has been a quantitative trait locus on bovine chromosome 6 (BTA6) found to be associated with milk yield, milk protein and fat percentage, which has been subsequently fine-mapped to six positional candidate genes. Subsequent investigations have yet to resolve which of the potential positional candidate genes is responsible for the observed associations with productive performance. In this study, we analysed candidate gene expression and the effects of gene knockdown on expression of ,- and ,-casein mRNA in a small interfering RNA transfected bovine in vitro mammosphere model. From our expression studies in vivo, we observed that four of the six candidates (ABCG2, SPP1, PKD2 and LAP3) exhibited differential expression in bovine mammary tissue over the lactation cycle, but in vitro functional studies indicate that inhibition of only one gene, SPP1, had a significant impact on milk protein gene expression. These data suggest that the gene product of SPP1 (also known as osteopontin) has a significant role in the modulation of milk protein gene expression. While these findings do not exclude other positional candidates from influencing lactation, they support the hypothesis that the gene product of SPP1 is a significant lactational regulatory molecule. [source] Genes differentially expressed in prostate cancerBJU INTERNATIONAL, Issue 8 2004I.E. Eder Because of the heterogeneity of prostate cancer knowledge about the genes involved in prostate carcinogenesis is still very limited. Previously, the use of novel high-throughput technologies offered the possibility to investigate broad gene expression profiles and thus helped to improve understanding of the molecular basis of prostate disease. Many candidate genes have been identified so far which have a more or less strong effect on prostate cancer. This vast number of gene expression changes show that it is unlikely that only one gene promotes prostate cancer. Conversely, it seems more likely that a broad network of molecular changes is involved in the complex cascade of events which lead to tumour formation and progression, respectively. A few of these novel molecular targets are currently under clinical evaluation. This paper gives an overview of several interesting candidate genes which may be useful as improved biomarkers for diagnosis or as targets for developing novel treatment methods. [source] Phenotype and genotype in females with POU3F4 mutationsCLINICAL GENETICS, Issue 6 2009S Marlin X-linked deafness is a rare cause of hereditary isolated hearing impairment estimated as at least 1% or 2% of the non-syndromic hearing loss. To date, four loci for DFN have been identified and only one gene, POU3F4 responsible for DFN3, has been cloned. In males, DFN3 is characterized by a progressive deafness associated with perilymphatic gusher at stapes surgery and with a characteristic inner ear malformation. The phenotype of eight independent females carrying POU3F4 anomalies is defined, and a late-onset hearing loss is found in three patients. Only one has an inner ear malformation. No genotype/phenotype correlation is identified. [source] | ||||||||||