Human Pathogenic Fungi (human + pathogenic_fungus)

Distribution by Scientific Domains

Selected Abstracts

Antimicrobial activity profiles of the two enantiomers of limonene and carvone isolated from the oils of Mentha spicata and Anethum sowa

K. K. Aggarwal
Abstract The antimicrobial activity of the essential oils of Mentha spicata L. and Anethum sowa Roxb. (Indian dill) were studied. The major chemical constituents of the hydrodistilled essential oils and their major isolates from cultivated M. spicata and A. sowa were identified by IR, 1H- and 13C-NMR and GC: (S)-(,)-limonene (27.3%) and (S)-(,)-carvone (56.6%) (representing 83.9% of the spearmint oil) and (R)-(+)-limonene (21.4%), dihydrocarvone (5.0%), (R)-(+)-carvone (50.4%) and dillapiole (17.7%) (together 76.9% in Indian dill oil), respectively. In vitro bioactivity evaluation of the isolated oil components revealed that both the optical isomers of carvone were active against a wide spectrum of human pathogenic fungi and bacteria tested. (R)-(+)-limonene showed comparable bioactivity profile over the (S)-(,)-isomer. The activity of these monoterpene enantiomers was found to be comparable to the bioactivity of the oils in which they occurred. Copyright © 2001 John Wiley & Sons, Ltd. [source]

Inhibition of clinical and environmental Cryptococcus neoformans isolates by two Brazilian killer yeasts

Alexandre M. Fuentefria
Two killer yeast strains (HB55 and HB88) capable of inhibiting human pathogenic fungi were isolated from leaves of Hibiscus rosa-sinensis in Brazil. These isolates were identified by conventional methods and sequencing of the D1/D2 region of the 26S rDNA as Kodamaea ohmeri . They inhibited all Cryptococcus neoformans (vars. neoformans , grubii and gattii ) strains tested, including reference, clinical and environmental isolates. The killer phenotype was not cured by thermal treatment. (© 2006 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim) [source]

New molecular methods to study gene functions in Candida infections

MYCOSES, Issue 9-10 2002
S. Theiß
Candida; Molekulargenetik; Genfunktion; Genexpression; ABC-Transporter Summary.Candida albicans has become a model system for human pathogenic fungi in clinical research, mainly due to the increasing number of Candida infections. Molecular techniques to study C. albicans virulence properties have been improved over the last few years, despite difficulties in genetic manipulation of this fungus. Some of the recent achievements from our own laboratory or from other groups are described in this article. The molecular analysis of the recently identified ATP-dependent transporter Mlt1 using the green fluorescent protein (GFP) as reporter for protein localization and the dominant MPAR gene as a selection marker for gene inactivation provides an example for the study of gene functions in C. albicans. Zusammenfassung.Candida albicans ist für die klinische Forschung zu einem Modellsystem zur Untersuchung humanpathogener Pilze geworden, was nicht zuletzt auf die steigende Zahl an Candida -Infektionen zurückzuführen ist. Trotz der Schwierigkeiten, die eine genetische Manipulation dieses Pilzes mit sich bringt, konnten in den letzten Jahren molekularbiologische Techniken zur Erforschung der Virulenzfaktoren von C. albicans weiterentwickelt werden. Einige dieser neuen Methoden, zum Teil aus unserer Arbeitsgruppe, aber auch aus anderen Laboratorien, werden in diesem Artikel beschrieben. Zudem gibt die Analyse des kürzlich isolierten, ATP-abhängigen Transporters Mlt1 ein Beispiel für die Studie von Genfunktionen, wobei das GFP (green fluorescent protein) als Reporter für Proteinlokalisation und der dominante Selektionsmarkers MPAR zur Geninaktivierung verwendet wurden. [source]

Synthesis and Antifungal Activity of 1-Aryl-3-phenethylamino-1-propanone Hydrochlorides and 3-Aroyl-4-aryl-1-phenethyl-4-piperidinols

Ebru Mete
Abstract Mono-Mannich bases, 1-aryl-3-phenethylamino-1-propanone hydrochlorides, 1a, 2a, 3a, 4a, 5a, 6a, 7a, 8a, 9a, and semi-cyclic mono-Mannich bases, 3-aroyl-4-aryl-1-phenethyl-4-piperidinols, 1b, 2b, 3b, 4b, 5b, 6b, 7b, 8b, 9b, were synthesized by a non-classical Mannich reaction. The aryl part was: C6H5 for 1a, 1b; 4-CH3C6H4 for 2a, 2b; 4-CH3OC6H4 for 3a, 3b; 4-ClC6H4 for 4a, 4b; 4-FC6H4 for 5a, 5b; 4-BrC6H4 for 6a, 6b; 2,4-(Cl)2C6H3 for 7a, 7b; 4-NO2C6H4 for 8a, 8b; and C4H3S(2-yl) i. e., 2-thienyl for 9a, 9b. Piperidinol compounds 2b, 3b, 4b, 5b, 7b, 8b, and 9b are reported here for the first time. The synthesized compounds were tested against seven types of plant pathogenic fungi and three types of human pathogenic fungi using the agar dilution assay. Itraconazole was tested against Candida parapsilosis as the reference compound, while Nystatin was tested as the reference compound against the other fungi. Compounds 1a, 1b, 2a, 4a, 4b, 5a, 5b, 6a, 7a, 8a, 9a, and 9b can be selected as model compounds to develop new antifungal agents against the human pathogen Microsporum canis. Compounds 8a and 8b, which had a similar antifungal activity compared with the reference compound Nystatin against the plant pathogen Aspergillus flavus, can serve as model compounds to develop new antifungal agents to solve agricultural problems. [source]